Human Interleukin 12B, IL12B ELISA Kit CKERS-IL12B-254H from Creative Diagnostics

Product Specs
ItemHuman Interleukin 12B, IL12B ELISA Kit
CompanyCreative Diagnostics
Price Supplier Page View Company Product Page
Catalog NumberCKERS-IL12B-254H
Quantity5plates
ApplicationsELISA
ReactivityHuman
SensitivityThe minimum detectable dose of human IL12B was determined to be approximately 3.91 pg/mL. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard
Detection TargetIL12B
Components1. Bring all reagents to room temperature before use.
2. Capture Antibody: 0.5 mg/mL of mouse anti-IL12B monoclonal antibody. Dilute to a working concentration of 1 µg/mL in CBS before coating.
3. Detection Antibody: Each vial contains 120 µg biotinylated rabbit anti-IL12B polyclonal antibody. Reconstitute with sterile 1 mL distilled water. Dilute to a working concentration of 1.5 µg/mL in detection antibody dilution buffer before use.
4. Standard: Each vial contains 3 ng of recombinant IL12B. Reconstitute standard power with sterile distilled water. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 250 pg/mL is recommended.
5. Streptavidin-HRP: 50 µL of streptavidin conjugated to horse-radish-peroxidase. 1:2,000 Dilution in detection antibody dilution buffer before use

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Human Interleukin 12B, IL12B ELISA Kit from Creative Diagnostics